Biochemical and Biophysical Research Communications, Vol.296, No.5, 1352-1355, 2002
Preparation and analysis of small unilamellar phospholipid vesicles of a uniform size
The interaction of carbonmonoxyhemoglobin and heme with small unilamellar phospholipid vesicles was studied using dynamic light scattering. Addition of carbonmonoxyhemoglobin to dimyristoylphosphatidylcholine:dimyristoylphosphatidylserine small unilamellar vesicles resulted in an increase of average vesicle size from 17.4 to 32.0 nm. Addition of heme to vesicles produced a smaller size increase, from 17.4 to 21.0 nm. Also reported is a method for preparing small unilamellar lipid vesicles of a uniform size, suitable for use in NMR spectroscopy. (C) 2002 Elsevier Science (USA). All rights reserved.