Biochemical and Biophysical Research Communications, Vol.294, No.2, 402-407, 2002
A novel and rapid approach to isolating functional ryanodine receptors
Conventional methods of isolating and reconstituting ryanodine receptors (RyRs) from native membranes into proteoliposomes take a minimum of 2 days to complete. We have developed an alternative strategy that can be used to isolate and reconstitute functional RyRs in just 3 h with a similar degree of purification. RyRs isolated by this method display characteristic functional behaviour as assessed by radioligand binding and single channel analyses. (C) 2002 Elsevier Science (USA). All rights reserved.