화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.289, No.2, 358-362, 2001
Involvement of tetrahydrobiopterin in trophic effect of erythropoietin on PC12 cells
Tetrahydrobiopterin (BH4) synthesis is reported to be stimulated by nerve growth factor (NGF) in PC12 cells, suggesting involvement of BH4 in the trophic effect of NGF. We have recently reported that erythropoietin (EPO) and BH4, enhance survival of PC12 cells. In the present study, we investigated involvement of BH4 in the trophic effect of EPO on PC 12 cells. Cellular BH4 content was increased by EPO (10(-10) to 10(-8) M) in a dose- and time-related manner. EPO (10(-10) to 10(-8) M) increased the viable cell number of PC12 cells. In addition to EPO, BH4 (1, 3, and 10 muM) increased the viable cell number of PC 12 cells. Administration of 0.3 mM 2,4-diamino-6-hydroxypyrimidine, an inhibitor of BH4 synthesis, blunted EPO-induced increases in BH4 content and the viable cell number of PC12 cells. These results taken together suggest that BH4 is involved in the trophic effects of EPO on PC12 cells.