화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.288, No.4, 893-900, 2001
Specific interaction between an oligosaccharide on the tumor cell surface and the novel antitumor agents, sulfoquinovosylacylglycerols
Some sulfoquinovosylacylglycerols (SQAG) have been shown to be potent DNA polymerase inhibitors, and to have strong antitumor activity in vivo. In this study, we investigated the mode of action of SQAG with regard to the interaction with the tumor cell surface. Of the SQAG used, the monoacyl forms (SQMG) with C18-, C18:1- or C16-fatty acids (SQMG-alpha C18, -alpha C18:1 or -alpha C16) effectively inhibited cell proliferation. of a hum an adenocarcinoma cell line, DLD-1, but SQMG-alpha C14 and the diacyl forms (SQDG) did not. Analysis of the interaction of SQMG-alpha C18 and -alpha C18:1 on three oligosaccharides of cell surface, sLe(A), Le(X), and SM3, by flow cytometry demonstrated that the A most effective interaction was observed on sLe(A) DLD-1 cells bound to SQMG-alpha C18:1-coated plates, and this binding was inhibited by monoclonal antibody against sLe(A) or SM3. However, these cells did not bind to SQMG-alpha C14-coated plates. Moreover the cytotoxic effects of SQMG-alpha C18, -alpha C18:1 on DLD-1 cells was inhibited by monoclonal antibodies against sLe(A) or SM3. Our results suggested that the interaction of SQMGs and sLe(A) plays an important role in suppression of the DLD-1 cell proliferation.