화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.274, No.3, 817-820, 2000
Study on colloidal Au-enhanced DNA sensing by quartz crystal microbalance
Colloidal Au is reported for enhancement the immobilization capacity and ultimately detection limit of DNA using quartz crystal microbalance (QCM). Immobilization of similar to 12 nm-diameter colloidal Au on to an An-coated QCM resulted in an easier attachment of oligonucleotide, with a mercaptohexyl group at the 5'-phosphate end and an increased capacity for nucleic acid detection, DNA immobilization and hybridization was monitored from QCM frequency changes, Hybridization was induced by exposure of the DNA-containing films to complementary DNA in solution, A much higher sensitivity was obtained for the analyte. The Au nanoparticle films on the Au plate provide a novel means for the fabrication of DNA sensor,