화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.268, No.2, 514-520, 2000
Activation of caspase-3 in molt4 cells by apoptosis-inducing nucleosides from CD57(+)HLA-DRbright natural suppressor cell line
Apoptosis-inducing nucleosides (ATNs), which were released and isolated from CD57(+)HLA-DRbright natural suppressor (57.DR-NS) cell line derived from human decidual tissue, induced apoptosis in Molt4 cells. The addition of caspase-3 inhibitor into the reaction blocked the cleavage of caspase-3 and apoptosis in Molt4 cells treated with AINs, detected by how cytometrical or spectrofluorometrical analysis and DNA fragmentation assay. Furthermore, by means of immunoblotting, the processing of caspase-3 was shown with the appearance of their catalytically active subunits of 20 and 11 kDa during the generation of apoptosis in Molt4 cells treated with AINs. This processing of caspase-3 into active subunits was also blocked by the addition of caspase-3 inhibitor. Thus, it was definitely revealed that the activation of caspase-3 was a key feature in the caspase cascade of AINs-induced apoptosis in Molt4 cells.