화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.267, No.1, 445-448, 2000
The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S-clavuligerus
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthase were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 reduced enzyme activity with penicillin N and penicillin G to different extents.