화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.350, No.3, 657-661, 2006
Association and insulin regulated translocation of hormone-sensitive lipase with PTRF
Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane. This localization was under control of insulin, which translocated PTRF to the cytosol and nucleus, indicating a novel role for PTRF in insulin transcriptional control. In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL). In response to insulin PTRF was translocated to the cytosol in parallel with HSL. PTRF and HSL were quantitatively immunoprecipitated from the cytosol by antibodies against either PTRF or HSL. The findings indicate also a novel extra-nuclear function for PTRF in the control of lipolysis. (c) 2006 Elsevier Inc. All rights reserved.