화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.345, No.3, 951-958, 2006
Activity-dependent regulation of beta-catenin via epsilon-cleavage of N-cadherin
N-cadherin is essential for excitatory synaptic contact in the hippocampus. Presenilin I (PSI) is located at sites of synaptic contact, forming a complex with N-cadherin and beta-catenin. Here, we report that human N-cadherin is cleaved by PSI/gamma-secretase in response to physiological concentration of glutamate (Glu) stimulation, yielding a fragment Ncad/CTF2. The expression of Ncad/CTF2 in neuronal cells led to its translocation to the nucleus, and caused a prominent enhancement of cytoplasmic and nuclear P-catenin levels in a cell-cell contact dependent manner, via following mechanisms: 1, inhibition of beta-catenin phosphorylation; 2, transactivation of beta-catenin; and 3, inhibition of N-cadherin transcription, and finally enhanced beta-catenin nuclear signaling. Since the regulation of cellular beta-catenin level is essential for synaptic function, disruption in the cleavage of N-cadherin may be causally linked to the synaptic dysfunction associated with Alzheimer's disease (AD). (c) 2006 Elsevier Inc. All rights reserved.