Biochemical and Biophysical Research Communications, Vol.342, No.3, 902-908, 2006
Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells
Bone morphogenetic proteins (BMPs) initiate, promote, and maintain chondrogenesis and osteogenesis. We hypothesize that BMP-2 induces an osteogenic, and BMP-7 a chondrogenic phenotype in adipose tissue-derived mesenchymal stem cells (AT-MSCs). We compared the effects of a short 15 min BMP-2 or BMP-7 (10 ng/ml) treatment on osteogenic and chondrogenic differentiation of AT-MSCs. Gene expression was studied 4 and 14 days after BMP-treatment. At day 4 BMP-2, but not BMP-7, stimulated runx-2 and osteopontin gene expression, and at day 14 BMP-7 down-regulated expression of these genes. At day 4 BMP-2 and BMP-7 stimulated biglycan gene expression, which was down-regulated by BMP-7 at day 14. BMP-7 stimulated aggrecan gene expression at day 14. Our data indicate that BMP-2 treatment for 15 min induces osteogenic differentiation, whereas BMP-7 stimulates a chondrogenic phenotype or AT-MSCs. Therefore, AT-MSCs triggered for only 15 min with BMP-2 or BMP-7 provide a feasible tool for bone and cartilage tissue engineering. (c) 2006 Elsevier Inc. All rights reserved.
Keywords:adipose tissue;mesenchymal stem cells;adipose derived stem cells;bone morphogenctic proteins;BMP-2;BMP-7;Runx-2;osteopontin;aggrecan