화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.340, No.3, 887-893, 2006
Association of fragile site-associated (FSA) gene expression with epithelial differentiation and tumor development
A novel gene designated as fragile site-associated (FSA) gene was recently identified by positional cloning from the CHO 1q31 fragile site which plays an important role in regulating amplification of multidrug resistance (mdrI) gene in inultidrug-resistant cells. FSA produces a message of similar to 16 kb which encodes an open-reading frame of 5005 amino acids. FSA shares sequence similarity with that in Caenorhabditis elegans 1pd-3, a lipid storage gene. Using immunohistochemical staining and RNA in Situ hybridization we report here that expression of FSA is associated with developmental programs of spermatogenesis and mammary gland in mice. Real-time RT-PCR results also support the upregulation of FSA expression in mammary gland development. Expression of FSA in many tissues including colon, skin, ovary, prostate, and bladder is mainly in the postmitotic, well-differentiated compartments. Moreover, levels of FSA expression are downregulated in tumours Of these tissue origins. These results suggest that FSA also plays important roles in regulating mammalian epithelial growth and differentiation and tumor development. (c) 2005 Elsevier Inc. All rights reserved.