화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.331, No.2, 476-483, 2005
Identification of non-mitochondrial NADPH oxidase and the spatio-temporal organization of its components in mouse spermatozoa
Though the sperniatozoa are known to produce Superoxide anion radicals, the enzyme systems(s) that produce Superoxide in these cells are not yet identified. Using Western blot assays and confocal laser scan microscopy. we detected gp91(phox) and p67(phox) associated with spermatozoa from testis and epididymis. We could not detect p22(phox) in ally of the sperm samples analyzed. While the expression of gp91(phox) p67(phox) appeared to be constitutive, p47(phox) was detectable only in spermatozoa from testis and vas deferens. In, Importantly, p40(phox) could be seen in very high quantities in testicular sperniatozoa. which also showed the highest levels of NADPH-oxidase activity. Spermatozoa from cauda epididymidis and as deferens also showed the presence of p40(phox), though the amount was low when compared with that of testicular spertilatozoa. The absence of p22(phox) and the striking correlation between the presence of p40(phox) and the NADPH-oxidase activity suggest that the NADPII oxidase associated with spermatozoa is p22(phox)-indepetident and that its activity is positively modulated by p40(phox). Further. Since the confocal imaging detected that the subunits of the NADPH oxidase are located significantly on the head domains, the sperrilato/oa appear to present a ease with dominant non-mitochondrial superoxide anion producing capabilities. © 2005 Elsevier Inc. All rights reserved.