화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.330, No.1, 186-193, 2005
New antibodies recognizing p73: Comparison with commercial antibodies
p73, unlike p53, is expressed as a number of isomeric forms. Alternative splicing at the 3' end of p73 transcript, together with the usage of a second promoter downstream of exon 3, can generate up to 24 p73 isoforms. Variants lacking the TA domain (AN isoforms) are induced by TAp73 and by p53, and inhibit their transcriptional activity. However, understanding the complex biology of p73 has been handicapped by the lack of high affinity specific antibodies for the different isoforms. Here, we report the characterization, by Western blotting and immunoprecipitation, of three new polyclonal antisera recognizing all p73 isoforms, only AN isoforms or only p73 alpha, and which have advantages of affinity and specificity over previously available antibodies. (c) 2005 Elsevier Inc. All rights reserved.