화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.323, No.3, 906-911, 2004
Stabilization of alanine substituted p53 protein at Ser15, Thr18, and Ser20 in response to ionizing radiation
Phosphorylation of p53 at Ser15, Thr18, and Ser20 has been thought to be important for p53 stabilization in response to ionizing radiation. In the present study, we examined the X-ray-induced stabilization of Ala-substituted p53 protein at Ser15, Thr18, and Ser20, whose gene expression was controlled under an ecdyson-inducible promoter. We found that all single-, double-, or triple-Ala-substituted p53 at Ser15, Yhr18, and Ser20 were accumulated in the nucleus similarly to wild-type p53 after X-irradiation. These results indicate that the phosphorylation of p53 at Ser15, Thr18, and Ser20 is not necessarily needed for p53 stabilization in response to ionizing radiation. (C) 2004 Elsevier Inc. All rights reserved.