화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.316, No.2, 317-322, 2004
Intermediate filament steady-state mRNA levels in amyotrophic lateral sclerosis
We have examined the steady-state levels of intermediate filament mRNA in amyotrophic lateral sclerosis using the RNAse protection assay (NFL, NFM, NFH; corrected against GAPDH) or by PCR (peripherin, alpha-internexin, nestin, and vimentin; corrected against beta-actin). Significant elevations of NFL and peripherin mRNA levels were observed within the ALS cervical and lumbar spinal cord, with all other IF mRNA levels being comparable between control and ALS cases. These findings suggest that disturbances in both NFL and peripherin expression, independently known to contribute to the generation of motor neuron dysfunction in transgenic mice, are evident in ALS. (C) 2004 Elsevier Inc. All rights reserved.