화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.315, No.4, 866-871, 2004
Conformational alteration of bradykinin in presence of GM1 micelle
We report here the interaction of bradykinin with ganglioside GM1 by circular dichroism, steady-state fluorescence, and one-dimensional H-1 NMR spectroscopy. Circular dichroism spectroscopy is indicative of a turn formation of bradykinin backbone in the presence of GM1 micelle. The fluorescence quenching efficiencies of iodide and acrylamide are substantially reduced, indicating a shielding of phenylalanine residue of bradykinin from aqueous environment. Significant line broadening of NMR resonances of bradykinin, suggestive of motional restriction, is observed. (C) 2004 Elsevier Inc. All rights reserved.