화학공학소재연구정보센터
Chinese Journal of Chemical Engineering, Vol.5, No.3, 219-225, 1997
Production of fructose-1,6-diphosphate (FDP) by permeabilized Saccharomyces cerevisiae and a kinetic model for FDP accumulation
Permeable yeast cells were used in the batch production of fructose-l, 6-diphosphate (FDP). The optimum reaction conditions were reported to be: reaction temperature 30 degrees C, toluene concentration 8% (V/V), and initial ratio of glucose to inorganic phosphorus (Pi) 10:1. Addition of AMP was found to be very beneficial to the FDP production. A multienzyme system model for FDP accumulation was developed, in which FDP was regarded as a substrate of phosphor-fructokinase (PFK), to simulate the activation effect of FDP on PFK. The model simulations were in good agreement with the experimental data.