화학공학소재연구정보센터
Macromolecular Research, Vol.15, No.1, 65-73, February, 2007
Preparation of a Porous Chitosan/Fibroin-Hydroxyapatite Composite Matrix for Tissue Engineering
E-mail:
Chitosan, fibroin, and hydroxyapatite are natural biopolymers and bioceramics that are biocompatible, biodegradable, and resorbable for biomedical applications. The highly porous, chitosan-based, bioceramic hybrid composite, chitosan/fibroin-hydroxyapatite composite, was prepared by a novel method using thermally induced phase separation. The composite had a porosity of more than 94% and exhibited two continuous and different morphologies: an irregularly isotropic pore structure on the surface and a regularly anisotropic multilayered structure in the interior. In addition, the composite was composed of an interconnected open pore structure with a pore size below a few hundred microns. The chemical composition, pore morphology, microstructure, fluid absorptivity, protein permeability, and mechanical strength were investigated according to the composition rate of bioceramics to biopolymers for use in tissue engineering. The incorporation of hydroxyapatite improved the fluid absorptivity, protein permeability, and tenacity of the composite while maintaining high porosity and a suitable microstructure.
  1. Drury JL, Mooney DJ, Biomaterials, 24, 4337 (2003)
  2. Khang G, Kim MS, Cho SH, Lee I, Rhee JM, Lee HB, Tissue Engineering and Regenerative Medicine, 1, 9 (2004)
  3. Steinbuchel A, Marchessault RH, Biopolymers for Medical and Pharmaceutical Applications, Wiley-VCH, Weinheim (2005)
  4. Hua FJ, Park TG, Lee DS, Polymer, 44(6), 1911 (2003)
  5. Guan J, Fujimoto KL, Sacks MS, Wagner WR, Biomaterials, 26, 3961 (2005)
  6. Jin HJ, Hwang MO, Yoon JS, Lee KH, Chin IJ, Kim MN, Macromol. Res., 13(1), 73 (2005)
  7. Daniels AU, Andriano KP, Smutz WP, Chang MKO, Heller J, J. Appl. Biomater., 5, 51 (1994)
  8. Li LH, Ding S, Zhou CR, J. Appl. Polym. Sci., 91(1), 274 (2004)
  9. Ellis DL, Yannas IV, Biomaterials, 17, 291 (1996)
  10. Zhao F, Yin Y, Lu WW, Leong JC, Zhang W, Zhang J, Zhang M, Yao K, Biomaterials, 23, 3227 (2002)
  11. Lahiji A, Sohrabi A, Hungerford DS, Frondoza CG, J. Biomed. Mater. Res., 51, 586 (2000)
  12. Vandevord PV, Matthew HWT, Desilva SP, Mayton L, Wu B, Wooley PH, J. Biomed. Mater. Res., 58, 585 (2002)
  13. Kim CH, Park HS, Gin YJ, Son YS, Lim SH, Choi YJ, Park KS, Park CW, Macromol. Res., 12(4), 367 (2004)
  14. Nishikawa H, Ueno A, Nishikawa S, Kido J, Ohishi M, Inoue H, Nagata T, J. Endodontics, 26, 169 (2000)
  15. Szuchet S, Watanabe K, Yamaguchi Y, Int. J. Dev. Neurosci., 18, 705 (2000)
  16. Suh JK, Matthew HW, Biomaterials, 21, 2589 (2000)
  17. Ishihara M, Nakanishi K, Ono K, Sato M, Kikuchi M, Saito Y, Yura H, Matsui T, Hattori H, Uenoyama M, Kurita A, Biomaterials, 23, 833 (2002)
  18. Freddi G, Monti P, Nagura M, Gotoh Y, Tsukada M, J. Polym. Sci. B: Polym. Phys., 35(5), 841 (1997)
  19. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y, J. Biomed. Mater. Res., 29, 1215 (1995)
  20. Chen X, Li WJ, Yu TY, J. Polym. Sci. B: Polym. Phys., 35(14), 2293 (1997)
  21. Kweon H, Ha HC, Um IC, Park YH, J. Appl. Polym. Sci., 80(7), 928 (2001)
  22. Kang GD, Lee KH, Ki CS, Nahm JH, Park YH, Macromol. Res., 12(5), 534 (2004)
  23. Kokubo T, Kim H, Kawashita M, Biomaterials, 24, 2161 (2003)
  24. Gomez-Vega JM, Saiz E, Tomsia AP, Marshall GW, Marshall SJ, Biomaterials, 21, 105 (2000)
  25. Rusu VM, Ng C, Wilke M, Tiersch B, Fratzl P, Peter MG, Biomaterials, 26, 5414 (2005)
  26. Miyamoto Y, Ishikawa K, Takechi M, Toh T, Yuasa T, Nagayama M, Suzuki K, Biomaterials, 19, 707 (1998)
  27. Murugan R, Ramakrishna S, Biomaterials, 25, 3829 (2004)
  28. Zahraoui C, Sharrock P, Bone, 25, 63 (1999)
  29. Muzzarelli RAA, Biagini G, DeBenedittis A, Mengucci P, Majni G, Tosi G, Carbon. Polym., 45, 35 (2001)
  30. Ma PX, Schloo B, Mooney D, Langer R, J. Biomed. Mater. Res., 29, 1587 (1995)
  31. Wintermantel E, Mayer J, Blum J, Eckert KL, Luscher P, Mathey M, Biomaterials, 17, 83 (1996)
  32. Baran ET, Tuzlakoglu K, Salgado AJ, Reis RL, J. Mater. Sci. -Mater. Med., 15, 161 (2004)
  33. Schugens C, Maquet V, Gradfils C, Jerome R, Teyssie P, J. Biomed. Mater. Res., 30, 449 (1996)
  34. Whinnery LL, Even WR, Beach JV, Loy DA, J. Polym. Sci. A: Polym. Chem., 34(8), 1623 (1996)
  35. Park SJ, Lee KY, Ha WS, Park SY, J. Appl. Polym. Sci., 74(11), 2571 (1999)
  36. Braier NC, Jishi RA, J. Mol. Struct., 499, 51 (2000)
  37. Yamaguchi I, Itoh S, Suzuki M, Osaka A, Tanaka J, Biomaterials, 24, 3285 (2003)
  38. Brine CJ, Sandford PA, Zikakis JP, Advances in Chitin and Chitosan, Elsevier Applied Science, London (1992)
  39. Ma PX, Langer R, in Polymers in Medicine and Pharmacy, Materials Research Society, Pittsburgh, 1995, pp 99-104
  40. Zhang Y, Zhang M, J. Biomed. Mater. Res., 55, 304 (2001)
  41. Shanmugasundaram N, Ravichandran P, Reddy PN, Ramamurty N, Pal S, Rao KP, Biomaterials, 22, 1943 (2001)
  42. Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R, J. Biomed. Mater. Res., 51, 475 (2000)
  43. Sailaja GS, Velayudhan S, Sunny MC, Sreenivasan K, Varma HK, Ramesh P, J. Mater. Sci., 38(17), 3653 (2003)
  44. Kim DK, Kim HS, Polym.(Korea), 29(4), 408 (2005)