화학공학소재연구정보센터
Biotechnology Letters, Vol.24, No.14, 1203-1207, 2002
An amperometric biosensor for determining amino acids using a bienzymatic system containing amino acid oxidase and protease
An amperometric biosensor for rapid determination of the concentration of L-amino acids has been developed using L-amino acid oxidase (L-AAO) immobilized by gel entrapment with poly( carbamoyl) sulfonate hydrogel. The broad substrate range of L-AAO allows this biosensor to be flexible in application. The artificial sweetener, aspartame, was determined by coupling L-AAO with pronase.