화학공학소재연구정보센터
Journal of Bioscience and Bioengineering, Vol.93, No.3, 318-321, 2002
Production of Ribonucleotides by autolysis of Hansenula anomala grown on Korean ginseng steaming effluent
Hansenula anomala KCCM 11473, which grew well in Korean ginseng steaming effluent, was selected for the production of 5'-ribonucleotides. This strain had high RNA content. Optimal autolysis conditions were established to produce 5'-ribonucleotides (13.9similar to28.5 mg/g of biomass) at 55degreesC and pH 5.0 for 24 h. 5'-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5'-ribonucleotides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase.