화학공학소재연구정보센터
Canadian Journal of Chemical Engineering, Vol.79, No.5, 732-736, 2001
Lag phase model for transient growth of Pseudomonas putida on phenol
The successful design of large-scale bioreactors requires the ability to predict both steady-state and dynamic operating conditions, At the same time mathematical models should not be too complex in order to reduce experimental work required to determine kinetic parameters. A simple model which predicts the behaviour of batch and transient continuous culture operations is presented and experimentally verified. The model is based on two regions of metabolic activity: the lag phase and the active phase. Pseudomonas putida growing on phenol as a substrate in a well-mixed bioreactor was tested in three modes of operation: batch, continuous start-up and continuous step-change. The model is demonstrated to predict all the qualitative aspects of the dynamic phases of growth and is quantitatively accurate.