화학공학소재연구정보센터
Journal of the Korean Industrial and Engineering Chemistry, Vol.12, No.6, 637-642, October, 2001
방사선 가교에 의한 젤라틴/키토산 하이드로겔과 PVP/젤라틴/키토산 하이드로겔의 제조 및 특성
Preparation and Characterization of Gelatin/Chitosan Hydrogel and PVP/Gelatin/Chitosan Hydrogel by Radiation Crosslinking
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초록
본 연구에서는, 방사선((60)Co γ-rays) 가교를 이용하여 키토산, 젤라틴, poly(N-vinylpyrrolidone)(PVP)의 혼합물로부터 하이드로겔(hydrogels)을 제조하였다. 하이드로겔이 상처 치료용 드레싱으로 사용될 수 있는지 예측하기 위해 겔화율, 팽윤도, 겔강도 같은 기계적 성질을 측정하였다. PVP와 젤라틴의 조성을 3:1로 하고, 이 PVP/젤라틴 용액과 젤라틴 용액의 고형분 농도는 20 wt%이었다. 젤라틴 또는 PVP/젤라틴 용액과 1 wt% 키토산 용액의 비는 25:75 ~ 75: 25이었다. 하이드로겔의 기계적 성질에 조사선량이 미치는 영향을 예측하기 위해 젤라틴/키토산 또는 PVP/젤라틴/키토산 혼합물에 25,35,50,60,70 kGy의 감마선을 조사하였다. 겔화율은 키토산 조성비가 작을수록 증가하였고, 조사선량이 커질수록 증가하였다. 팽윤도는 키토산 조성비가 커질수록 증가하고, 조사선량이 작을수록 증가하였다. 팽윤도는 겔화가 증가할수록 가교 밀도가 증가하기 때문에 겔화율에 반비례한다. 겔강도는 키토산 조성비가 작을수록 증가하였고, 조사선량이 증가할수록 커졌다. 하이드로겔이 상업용 바셀린 거즈보다 치료효과가 우수하였다.
Hydrogels from a mixture of chitosan, gelatin and poly(N-vinylpyrrolidone)(PVP) were made by γ-ray irradiation. The mechanical properties of gelatin, water absorptivity, and gel strength were examined to evaluate application of the hydrogels for a wound dressing. The ratio of composition of PVP to gelatin was 3:1, and the solid concentration of PVP/gelatin in the gelatin solution was 20 wt%. The ratio of gelatin or PVP/gelatin solution to 1 wt% chitosan solution was in the range of 25:75 ~ 75:25. Gamma irradiation doses of 25,35,50,60 and 70 kGy were exposed to the mixture of gelatin/chitosan of PVP/gelatin/chitosan to evaluate the effect of irradiation dose on the mechanical properties of hydrogels. The results showed that gel content and gel strength increased as the chitosan concentration in gelatin/chitosan or PVP/gelatin/chitosan decreased, and it increased as irradiation dose increased. Swelling percent increased as the chitosan concentration in gelatin/chitosan or PVP/gelatin/chitosan increased, but it increased as irradiation dose decreased. The swelling percent was inversely proportional to the gel percent because the cross-linking density increases with the gelation. This study showed that using the hydrogel for a wound dressing had a better curing effect than a common vaseline gauze.
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