Biotechnology Letters, Vol.20, No.10, 943-947, 1998
Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli
Approximately 30% of rice hulls, which represent an abundant residue with little commercial value, was solubilized with 0.4 M H2SO4 acid to produce a syrup containing over 100 g monomer sugar/l. Toxins generated during hydrolysis were mitigated with Ca(OH)(2). Treated hydrolysate plus additional nutrients was fermented with Escherichia coli KO11 to produce over 46 g ethanol/L in 72 h (92% of theoretical yield).