화학공학소재연구정보센터
Biotechnology Letters, Vol.23, No.8, 643-646, 2001
High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector
Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg l(-1) of culture.