화학공학소재연구정보센터
Enzyme and Microbial Technology, Vol.28, No.4-5, 329-332, 2001
Preparation and catalytic performance of surfactant-manganese peroxidase-Mn-II ternary complex in organic media
A novel preparation method for surfactant-MnP-Mn-II ternary complex utilizing water-in-oil emulsions has been developed. The surfactant-MnP complex was spectroscopically characterized, strongly suggesting that the heme environment of the surfactant-MnP complex in benzene is identical to that of native MnP in the aqueous buffer. o-Phenylenediamine oxidation catalyzed by the surfactant-MnP-Mn-II ternary complex was performed in benzene. The ternary complex efficiently catalyzed the oxidation, and the complex was catalytically stable. Kinetic experiments revealed that the reaction mechanism was as follows: MnP is oxidized by H2O2 and the oxidized intermediate catalyzes the oxidation of Mn-II to Mn-III and the latter, after complexed with malonate, readily oxidizes o-PDA inside the complex. Thus, the organic substrate U-PDA, but not Mn-III, shuttled between the surfactant-MnP-Mn-II ternary complex and organic solvent. (C) 2001 Elsevier Science Inc. All rights reserved.