Biotechnology and Bioengineering, Vol.44, No.10, 1186-1192, 1994
A Dense Cell Retention Culture System Using a Stirred Ceramic Membrane Reactor
A novel reactor design incorporating porous ceramic tubes into a stirred jar fermentor was developed. The stirred ceramic membrane reactor has two ceramic tubular membrane units inside the vessel and maintains high filtration flux by alternating use for filtering and recovering from clogging. Each filter unit was linked for both extraction of culture broth and gas sparging. High permeability was maintained for long periods by applying the periodical control between filtering and air sparging during the stirred retention culture of Saccharomyces cerevisiae. The ceramic filter aeration system increased the k(L)a to about five times that of ordinary gas sparging. Using the automatic feeding and filtering system, cell mass concentration reached 207 g/L in a short time, while it was 64 g/L in a fed-batch culture. More than 99% of the growing cells were retained in the fermenter by the filtering culture. Both yield and productivity of cells were also increased by controlling the feeding of fresh medium and filtering the supernatant of the dense cell culture.
Keywords:FED-BATCH CULTURE;DEPENDENT ANIMAL-CELLS;CROSS-FLOW FILTRATION;MASS-PRODUCTION;FERMENTATION;ADHESIVENESS;SUBSTRATE;CARRIERS;ETHANOL;OXYGEN