화학공학소재연구정보센터
Separations Technology, Vol.4, No.4, 258-260, 1994
PHASE-SEPARATION OF ACETONITRILE-WATER MIXTURE IN PROTEIN-PURIFICATION
The removal of acetonitrile (ACN) from reversed-phase high performance liquid chromatography (RP-HPLC) effluent fractions often presents a problem. High concentrations of ACN place a tough demand on the equipment in terms of solvent resistance. Energy cost is also a concern when ACN is removed by evaporation or freeze-drying. This work shows that a phase separation occurs for ACN-water solutions at -17-degrees-C. The top phase contains 88 (volume)% ACN, and the bottom phase is 65% water. Since the bottom phase contains 35% ACN, it is not frozen. Surprisingly, proteins such as human growth hormone and its analogs remain in the bottom phase 99% or more after a phase separation. This appears to be an easy and energy efficient method to remove the majority of ACN after RP-HPLC.