Science, Vol.266, No.5186, 789-793, 1994
Isolation of the Tomato Cf-9 Gene for Resistance to Cladosporium-Fulvum by Transposon Tagging
The tomato Cf-9 gene confers resistance to infection by races of the fungus Cladosporium fulvum that carry the avirulence gene Avr9. The Cf-9 gene was isolated by transposon tagging with the maize transposable element Dissociation, The DNA sequence of Cf-9 encodes a putative membrane-anchored extracytoplasmic glycoprotein. The predicted protein shows homology to the receptor domain of several receptor-like protein kinases in Arabidopsis, to antifungal polygalacturonase-inhibiting proteins in plants, and to other members of the leucine-rich repeat family of proteins. This structure is consistent with that of a receptor that could bind Avr9 peptide and activate plant defense.
Keywords:PLATELET GLYCOPROTEIN-IB;LEUCINE-RICH REPEATS;PROTEIN-KINASE;TRANSMEMBRANE PROTEIN;DISEASE-RESISTANCE;ARABIDOPSIS-THALIANA;RECEPTOR;CLONING;DOMAIN;SEQUENCE