화학공학소재연구정보센터
Applied Microbiology and Biotechnology, Vol.43, No.3, 529-533, 1995
Degradation of Mixtures of Chloroaromatic Compounds in Soil Slurry by Mixed Cultures of Specialized Organisms
The degradation of a mixture of 13 chloroaromatics, 2-chloro-, 3-chloro-, 4-chloro- and 3,4-dichloroaniline, 2-chloro-, 3-chloro-, 4-chloro-, 3,4-dichloro- and 3,5-dichlorobenzoate, and chloro-, 1,2-dichloro-, 1,4-dichloro- and 1,2,4-trichlorobenzene in soil slurries by a mixed culture of Pseudomonas acidovorans strain BN 3.1, Pseudomonas ruhlandii strain FRB2, Pseudomonas cepacia strain JH230 and Pseudomonas aeruginosa strain RHO1 was studied. About 70% of the organic bound chlorine was eliminated after 25 days from soil with a carbon content of 8% (soil 1) when 2-3 x 10(5) cells/g soil of each of the strains were added to the slurries. The effect of the clean-up was demonstrated by a biological test using cress and wheat. Both plants showed good germination and growth on both non-contaminated soils and the contaminated soil 1 after the biotreatment with the strains. No growth was observed when the plants were incubated with the contaminated soil 1 and with the contaminated and biotreated soil 2 (carbon content 2.6%). This indicates that the remaining 30% of organic chlorine in soil 1 after biotreatment does not influence the germination and growth of the two plants tested.