2,5-Furandicarboxylic acid (FDCA) is non-toxic, biodegradable, and structurally similar to aromatic di-acids derived from petroleum (petroleum-based aromatic di-acids). In this paper, the polyamide (POF) of FDCA and 4,4′-diaminodiphenyl ether was synthesized in presence of LiCl and N,N-dimethylformamide, and then the new POF membrane was prepared from POF polymer by non-solvent-induced phase separation (NIPS) method. The surface morphology, roughness, contact angle, and zeta potential of the POF membrane surface were well investigated. The results showed that the POF membrane was a hydrophilic, negatively charged, and hard brittle material with high tensile strength. The effects of different POF concentrations in membrane casting solution on the performance of POF membrane were studied through the characterization of flux and interception. When POF concentration was 20 wt%, the prepared membrane exhibited the best performance in long-term stability test, and the flux was 43.9 L m-2 h-1 bar-1, and the rejection rates of the POF membrane for Evans blue, Congo red, and naphthol green B were 95.6%, 93.7%, and 89%, respectively.