화학공학소재연구정보센터
Journal of Industrial and Engineering Chemistry, Vol.114, 171-180, October, 2022
Glutathione-depleted and cancer-targeted nanocapsules encapsulating bimetallic oxide nanoparticles for enhanced chemo-sonodynamic therapy
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Sonodynamic therapy (SDT) has emerged as a promising noninvasive therapeutic modality due to its deep tissue penetration depth. Herein, biodegradable poly(lactic-co-glycolic acid) (PLGA) nanocapsules encapsulating oxygen-deficient MnWOx nanoparticles (NPs) and doxorubicin (DOX) were fabricated for chemo-sonodynamic combination therapy against cancer. To achieve cancer-targeted chemo-SDT, PLGA was conjugated to a cancer-targeting biotin through poly(ethylene glycol) (PEG). Biotin-PEG-PLGA (BP-PLGA) nanocapsules encapsulating DOX and hydrophobic MnWOx NPs (BP-PLGA-DOX@MnWOx) exhibited high physiological stability and pH-responsive drug release. The oxygen-deficient MnWOx NPs enabled US-triggered generation of singlet oxygen and hydroxyl radicals owing to their oxygen-deficient structures that prevent electron–hole recombination. Glutathione (GSH) depletion by MnWOx-loaded PLGA nanocapsules was observed in MCF-7 human breast cancer cells, which leads to augmented intracellular ROS levels and sonodynamic effects. Notably, BP-PLGA-DOX@MnWOx greatly improved cellular uptake by breast cancer cells, resulting in efficient intracellular delivery of DOX and MnWOx NPs. As a result, BP-PLGA-DOX@MnWOx exhibited efficient and cancer-targeted cytotoxicity in MCF-7 cells upon US irradiation. This study demonstrates that BPPLGA- DOX@MnWOx nanocapsules are promising cancer-targeting nanosonosensitizers for efficient chemo-sonodynamic cancer therapy.
  1. Yan P, Liu LH, Wang P, ACS Appl. Bio Mater., 3(6), 3456 (2020)
  2. Qian X, Zheng Y, Chen Y, Adv. Mater., 28(37), 8097 (2016)
  3. Pan X, Bai L, Wang H, Wu Q, Wang H, Liu S, Xu B, Shi X, Liu H, Adv. Mater., 30(23), 1800180 (2018)
  4. Kennedy JE, Nat. Rev. Cancer, 5(4), 321 (2005)
  5. Lafond M, Umemura SI, Yoshizawa S, J. Ultrasound Med., 38(3), 567 (2019)
  6. Huang J, Liu F, Han X, Zhang L, Hu Z, Jiang Q, Wang Z, Ran H, Wang D, Li P, Theranostics, 8(22), 6178 (2018)
  7. Son S, Kim JH, Wang X, Zhang C, Yoon SA, Shin J, Sharma A, Lee MH, Cheng L, Wu J, Kim JS, Chem. Soc. Rev., 49(11), 3244 (2020)
  8. Lin X, Song J, Chen X, Yang H, Angew. Chem.-Int. Edit., 59(34), 14212 (2020)
  9. Xing X, Zhao S, Xu T, Huang L, Zhang Y, Lan M, Lin C, Zheng X, Wang P, Coord. Chem. Rev., 445 (2021)
  10. Sun L, Wang P, Zhang J, Sun Y, Sun S, Xu M, Zhang L, Wang S, Liang X, Cui L, Biomater. Sci., 9(6), 1945 (2021)
  11. Han X, Huang J, Jing X, Yang D, Lin H, Wang Z, Li P, Chen Y, ACS Nano, 12(5), 4545 (2018)
  12. Deepagan VG, You DG, Um W, Ko H, Kwon S, Choi KY, Yi GR, Lee JY, Lee DS, Kim K, Kwon IC, Park JH, Nano Lett., 16(10), 6257 (2016)
  13. Su K, Tan L, Liu X, Cui Z, Zheng Y, Li B, Han Y, Li Z, Zhu S, Liang Y, Feng X, Wang X, Wu S, ACS Nano, 14(2), 2077 (2020)
  14. Gong F, Cheng L, Yang N, Betzer O, Feng L, Zhou Q, Li Y, Chen R, Popovtzer R, Liu Z, Adv. Mater., 31(23), 1900730 (2019)
  15. Dong Y, Dong S, Liu B, Yu C, Liu J, Yang D, Yang P, Lin J, Adv. Mater., 33(51), 2106838 (2021)
  16. Pan D, Caruthers SD, Senpan A, Schmieder AH, Wickline SA, Lanza GM, WIREs Nanomed. Nanobiotechnol., 3(2), 162 (2011)
  17. Quan Z, Ruowei T, Zhao ZHX, Jiang J, Li J, Fu YY, ACS Appl. Nano Mater., 1(1), 101 (2018)
  18. Fredenberg S, Wahlgren M, Reslow M, Axelsson A, Int. J. Pharm., 415(1-2), 34 (2011)
  19. Purushothaman B, Lee J, Hong S, Song JM, J. Nanobiotechnol., 18(1), 102 (2020)
  20. Lu S, Feng W, Dong C, Song X, Gao X, Guo J, Chen Y, Hu Z, Adv. Healthcare Mater., 11(3), 2102135 (2022)
  21. Hong EJ, Lee D, Kang HC, Kim YC, Shim MS, J. Ind. Eng. Chem., 63, 57 (2018)
  22. Hong EJ, Sivakumar P, Ravichandran V, Choi DG, Kim YS, Shim MS, ACS Biomater. Sci. Eng., 5(10), 5209 (2019)
  23. Zhao J, Li J, Wang Y, Zhao J, Yang H, Wang X, Sci. Rep., 7(1), 18014 (2017)
  24. Liang C, Li N, Cai Z, Liang R, Zheng X, Deng L, Feng L, Guo R, Wei B, Artif. Cells Nanomed. Biotechnol., 47(1), 4211 (2019)
  25. Makadia HK, Siegel SJ, Polymer, 3(3), 1377 (2011)
  26. Mir M, Ahmed N, Rehman AU, Colloids Surf. B: Biointerfaces, 159, 217 (2017)
  27. Cheng J, Teply BA, Sherifi I, Sung J, Luther G, Gu FX, Levy-Nissenbaum E, Radovic-Moreno AF, Langer R, Farokhzad OC, Biomaterials, 28(5), 869 (2007)
  28. Shapiro EM, Koretsky AP, Magn. Reson. Med., 60(2), 265 (2008)
  29. Bennewitz MF, Lobo TL, Nkansah MK, Ulas G, Brudvig GW, Shapiro EM, ACS Nano, 5(5), 3438 (2011)
  30. de la Torre CM, Grossman JH, Bobko AA, Bennewitz MF, PLoS One, 15(9) (2020)
  31. Teranishi R, Matsuda T, Yuba E, Kono K, Harada A, Macromol. Biosci., 19(4) (2019)
  32. Xia C, Zeng H, Zheng Y, Mol. Med. Rep., 21(3), 989 (2020)
  33. Dong Z, Feng L, Hao Y, Li Q, Chen M, Yang Z, Zhao H, Liu Z, Chem, 6(6), 1391 (2020)
  34. Lin LS, Song J, Song L, Ke K, Liu Y, Zhou Z, Shen Z, Li J, Yang Z, Tang W, Niu G, Yang HH, Chen X, Angew. Chem.-Int. Edit., 57(18), 4902 (2018)
  35. Hoang QT, Ravichandran V, Cao TGN, Kang JH, Ko YT, Lee TI, Chem. Eng. J., 435(3) (2022)