Journal of Bioscience and Bioengineering, Vol.130, No.3, 233-238, 2020
Cloning and functional analysis of AmDUF1517 promoter from Ammopiptanthus mongolicus
Domains of unknown function protein family 1517 (DUF1517) in Ammopiptanthus mongolicus, could be induced by abiotic stresses, whose upstream regulatory sequence might be an ideal source of abiotic-induced promoter. In this study, a 1026-bp promoter of AmDUF1517 from A. mongolicus was cloned. Five deletion fragments (Full, Q1-Q4) of different length of the AmDUF1517 promoter were fused with the beta-glucuronidase (GUS) reporter and transformed into Arabidopsis thaliana. The deletion analysis showed that sequences Full, Q1 and Q3 responded well to mannitol, NaCl and 4 degrees C stresses, while Q2 and Q4 segments did not. The Q3 fragment (280 bp; -280 to -1 bp) showed the highest promoter activity under normal and mannitol, NaCl and 4 degrees C conditions. The result suggested that Q3 in the AmDUF1517 gene promoter could be a new source of induced promoters for abiotic resistance breeding in plant genetic engineering. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.