화학공학소재연구정보센터
Industrial & Engineering Chemistry Research, Vol.59, No.47, 20646-20655, 2020
Design and Construction of Enzyme-Nanozyme Integrated Catalyst as a Multifunctional Detection Platform
A new integrated catalyst, Fe3O4@Cu/GMP-GO(x), was successfully constructed by assembling Cu2+ and guanosine 5-monophosphate (GMP) on magnetic nanoparticles and in situ encapsulating glucose oxidase (GOx). As the nanozyme, Fe3O4@Cu/GMP has the dual response of catalase- and laccase-like activity, which can effectively improve the cascade catalytic efficiency and amplify the colorimetric detection signal. Compared with free enzymes, Fe3O4@Cu/GMP and Fe3O4@Cu/GMP-GO(x) showed enhanced thermal, mechanical, and storage stability. Fe3O4@Cu/GMP can be applied to the detection of H2O2 , with a linear response range of 0 to 198 mu M and a limit of detection (LOD) of 0.477 mu M. Fe3O4@Cu/GMP-GO(x) can be applied to the detection of glucose and beta-arbutin and showed good selectivity and anti-interference performance. For glucose, the linear response range was 0 to 800 mu M, and the LOD was 5.516 mu M. For arbutin, the absorbance was linearly correlated with the concentration of arbutin in the range of 0 to 60 mu M and 100 to 400 mu M, and the LOD was 0.766 mu M. This integrated catalyst provides a simple strategy for the application of an enzyme-nanozyme cascade system in colorimetric detection.