화학공학소재연구정보센터
Biomacromolecules, Vol.21, No.9, 3608-3619, 2020
Controlling Drug Absorption, Release, and Erosion of Photopatterned Protein Engineered Hydrogels
A protein-engineered triblock copolymer hydrogel composed of two self-assembling domains (SADs) has been fabricated by a photoactivatable diazirine group followed by ultraviolet (UV)-mediated crosslinking. The photocrosslinkable protein polymer CEC-D has been patterned into various features including different micrometer-scale stripes by using lithographic techniques. The patterned hydrogels are important for encapsulation of small molecules where a photopatterned fraction of 50% is optimal for maximum absorption. Stripe-patterned CEC-D100-100 exhibits slightly lower swelling ratios, an 8.9 times lower erosion profile, and a 2.6-fold higher drug release compared to the unpatterned hydrogel control, CEC-D-0. Our studies demonstrate the potential of photocrosslinkable protein polymer hydrogels to be used as scaffolds for therapeutic delivery of small molecules. Through photolithographic techniques on the protein hydrogel, variety of functionalities can be achieved by patterning different features enabling the mimicry of biological systems.