화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.531, No.1, 62-66, 2020
Stapling a G-quadruplex specific peptide
G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure and the RHAU helicase has been identified to have high specificity for recognition of parallel-stranded G4s. We have designed and synthesized two stapled peptide analogues of the G4-specfic motif of RHAU, which preserve the G4 binding ability. Characterization of these peptides identified the stapled variants to exhibit higher helical formation propensity in aqueous buffer in comparison to the native RHAU sequence. Moreover, the stapled peptides exhibit superior enzymatic stability towards alpha-chymotrypsin. Our stapled RHAU peptides can serve as a new tool for targeting G4 nucleic acid structures . (c) 2020 Elsevier Inc. All rights reserved.