화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.532, No.1, 127-133, 2020
Efficient screening of ligand-receptor complex formation using fluorescence labeling and size-exclusion chromatography
Evidence of a complex formation is a crucial step in the structural studies of ligand-receptor interactions. Here we presented a simple and fast approach for qualitative screening of the complex formation between the chimeric extracellular domain of the nicotinic acetylcholine receptor (alpha 7-ECD) and threefinger proteins. Complex formation of snake toxins alpha-Bgtx and WTX, as well as of recombinant analogs of human proteins Lynxl and SLURP-I, with alpha 7-ECD was confirmed using fluorescently labeled ligands and size-exclusion chromatography with simultaneous absorbance and fluorescence detection. WTX/alpha 7-ECD complex formation also was confirmed by cryo-EM. The proposed approach could easily be adopted to study the interaction of other receptors with their ligands. (C) 2020 Elsevier Inc. All rights reserved.