화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.533, No.4, 945-951, 2020
Lysine is required for growth factor-induced mTORC1 activation
Mechanistic target of rapamycin complex 1 (mTORC1) integrates various environmental signals to regulate cell growth and metabolism. mTORC1 activity is sensitive to changes in amino acid levels. Here, we investigated the effect of lysine on mTORC1 activity in non-small cell lung cancer (NSCLC) cells. Lysine deprivation suppressed mTORC1 activity and lysine replenishment restored the decreased mTORC1 activity in lysine-deprived cells. Supplementing growth factors, such as insulin growth factor-1 or insulin restored the decreased mTORC1 activity in serum-deprived cells. However, in serum/lysine-deprived cells, supplementing growth factors was not sufficient to restore mTORC1 activity, suggesting that growth factors could not activate mTORC1 efficiently in the absence of lysine. General control non-derepressible 2 and AMP-activated protein kinase were involved in lysine deprivation-mediated inhibition of mTORC1. Taken together, these results suggest that lysine might play role in the regulation of mTORC1 activation in NSCLC cells. (C) 2020 Elsevier Inc. All rights reserved.