Applied Microbiology and Biotechnology, Vol.104, No.7, 2897-2909, 2020
Impact and relevance of alcohol dehydrogenase enantioselectivities on biotechnological applications
Alcohol dehydrogenases (ADHs) catalyze the reversible reduction of a carbonyl group to its corresponding alcohol. ADHs are widely employed for organic synthesis due to their lack of harm to the environment, broad substrate acceptance, and high enantioselectivity. This review focuses on the impact and relevance of ADH enantioselectivities on their biotechnological application. Stereoselective ADHs are beneficial to reduce challenging ketones such as ketones owning two bulky substituents or similar-sized substituents to the carbonyl carbon. Meanwhile, in cascade reactions, non-stereoselective ADHs can be utilized for the quantitative oxidation of racemic alcohol to ketone and dynamic kinetic resolution.
Keywords:Alcohol dehydrogenase;Enantioselectivity;Asymmetric reduction;Enzyme engineering;Cascade;Pharmaceutical intermediate