Applied Biochemistry and Biotechnology, Vol.193, No.2, 577-588, 2021
Enhancement of Galactose Uptake fromKappaphycus alvareziiUsingSaccharomyces cerevisiaethrough Deletion of Negative Regulators ofGALGenes
This study was aimed at enhancing galactose consumption from the red seaweedKappaphycus alvarezii.The optimal pretreatment condition of thermal acid hydrolysis was treated with 350 mM HNO(3)for 60 min at 121 degrees C. The enzymatic saccharification with a 1:1 mixture of Celluclast 1.5 L and Viscozyme L showed the maximum yield of glucose; 42-g/L monosaccharide concentration was obtained with the highest yield of pretreatment and enzymatic saccharification (E-PS) and the lowest inhibitory compound concentration. The deletion of theGAL80,MIG1,CYC8, orTUP1gene was performed to improve the galactose consumption rate. The strains with the deletion of theMIG1gene (mig1 Delta) showed higher galactose consumption rate and ethanol yield than other strains. High transcription levels of regulatory genes revealed that themig1 Delta relieved glucose repression. These results show that themig1 Delta enhances galactose consumption rate fromK. alvarezii.