화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.529, No.2, 425-431, 2020
Fatty acids influence the efficacy of lutein in the modulation of alpha-crystallin chaperone function: Evidence from selenite induced cataract rat model
Background: Loss of alpha-crystallin chaperone function results in the lens protein aggregation leading to cataract. In this study, we evaluated the efficacy of micellar lutein with different fatty acids in modulating alpha-crystallin chaperone function under selenite cataract conditions. Methods: Cataract was induced in rat pups by giving sodium selenite (25 mu M/kg body weight) by IP. Lutein [(L), 1.3 mu mol/kg body weight)] was given day before and five days after selenite injection as a micelle with 7.5 mM linoleic acid (LA), or 7.5 mM eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA) or 7.5 mM oleic acid (OA). Lens alpha-crystallins was purified, and its chaperone function and integrity was assessed. Cholesterol, calcium, calpain-2, procaspase-3, and expression of alpha-A and beta-B1 crystallin in the lens of cataract and micellar lutein administered rats were evaluated. Results: Cataract induction significantly (p < 0.05) decreased lens alpha-crystallin chaperone function. Cataract rats had increased cholesterol and calcium level, increased the expression of calpain-2, and alpha-A and beta-B1 crystallin, and reduced the pro-caspase-3 level in the lens. However, micellar lutein administration significantly (p < 0.05) protected client proteins from aggregation via the modulation of calcium-dependent calpain-2 protease activity. The chaperone function of lens alpha-crystallins in rats administered micellar lutein with EPA + DHA was found to be highest when compared to OA and LA. Conclusions: Micellar lutein with unsaturated fatty acids beneficially modulates alpha-crystallin chaperone function. Among the fatty acids tested, micellar lutein with EPA + DHA exhibited superior effects, thereby offering a promising strategy for cataract management. (C) 2020 Elsevier Inc. All rights reserved.