화학공학소재연구정보센터
Advanced Powder Technology, Vol.31, No.3, 1323-1332, 2020
Green synthesized silver nanoparticle from Allium ampeloprasum aqueous extract: Characterization, antioxidant activities, antibacterial and cytotoxicity effects
In the current study for the first time, silver nanoparticles (AgNPs) were biosynthesized by reducing agents from hot water extract of Allium ampeloprasum, an antibacterial and anti-inflammatory edible plant. UV-vis. spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering, X-ray diffractometric, and transmission electron microscopy (TEM) analyses have been applied to confirm the formation of biosynthesized AgNPs. Total phenol content and antioxidant activities of AgNPs and extract together with their antibacterial and cytotoxic properties, were evaluated. According to TEM, AgNPs were spherical with a diameter of 8-50 nm. Total phenolic compounds were 15.58 mu g/mL, and 10.94 mu g/mL at a concentration of 150 mu g/mL for the A. ampeloprasum extract and the biosynthesized AgNPs, respectively. Biosynthesized AgNPs showed significant antioxidant activity (81%) as compared to A. ampeloprasum extract (32%) and were active on multi-drug resistant P. aeruginosa. Besides, the cytotoxic activity response was also demonstrated that AgNPs were more potent than the A. ampeloprasum extract and showed high activity against Hela cell line with an IC50 value of less than 25 mu g/mL. In conclusion, AgNPs synthesized by A. ampeloprasum extract with excellent antioxidant and antibacterial effects and acceptable cytotoxicity on cervical cancer cells have the potential to be used in biological applications. (C) 2020 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.