Bioactivity exhibited by nanoparticles has been interesting for researchers globally. The phytogenic synthesis of zinc oxide nanoparticles (ZnO-NPs) has been an eco-friendly approach due to its low toxicity and biological potential. In this pretext, the present study has been conducted. The bacterial inhibition by ZnO-NPs could be attributed to its high specific surface charge and reactive oxygen species generation. The present study states the phyto-fabrication of ZnO-NPs employing aqueous leaf extract of Canthium dicoccum (L.). The synthesized nanoparticles (NPs) displayed characteristic excitation at 293 nm. The dynamic light scattering (DLS) analysis revealed an average 33 nm size of ZnO-NPs. The FT-IR functional groups C-H stretch, C-H bend, aromatic C-N stretch, and C-O stretch were observed in phyto-fabricated ZnO-NPs. Results obtained from antibacterial activities inferred that ZnO-NPs were most effective against Bacillus subtilis, least sensitive to Staphylococcus aureus. The minimum inhibitory concentration (MIC) was observed in the range 78.12 to 625 mu g mL(-1) which was further confirmed by bacterial cell viability test. The Anti-TB activity by Alamar Blue Dye test revealed phytofabricated ZnO-NPs inhibited M. tuberculosis at 25 mu g mL(-1). The result of the antioxidant activity of the DPPH method was dose-dependent. The application of ZnO-NPs as potential antibacterial applications could be envisioned.