화학공학소재연구정보센터
Journal of Applied Microbiology, Vol.128, No.4, 1179-1190, 2020
The synergy of resveratrol and alcohol against Helicobacter pylori and underlying anti-Helicobacter pylori mechanism of resveratrol
Aims To determine individual antibacterial and synergistic antibacterial effects of resveratrol and alcohol against Helicobacter pylori 26695 in vitro, and to elucidate the underlying mechanism of action of resveratrol against H. pylori. Methods and Results The minimum inhibitory concentrations (MICs) and time-killing curve of resveratrol and alcohol were determined. Transcriptome analysis by RNA sequencing was used to elucidate the underlying mechanism of action of resveratrol against H. pylori. Our results showed that the MICs of resveratrol and alcohol against H. pylori 26695 are about 64 mu g ml(-1) and 4% (v/v) respectively. The synergy was found: resveratrol at concentration of 64 mu g ml(-1) in combination with alcohol at concentration of 4% (v/v) showed >10 000-fold decrease in the mount of viable bacteria compared with resveratrol and alcohol used alone. Transcriptome analysis showed 152 genes were downregulated and 111 genes were upregulated in the presence of resveratrol. Genes involved in protein translation (17 center dot 1%), outer membrane proteins (OMPs) (9 center dot 9%) and transports (11 center dot 2%) comprise 38 center dot 2% of the downregulated genes. In comparison, genes involved in redox (13 center dot 5%), pathogenesis and motility (9 center dot 9%) and iron homeostasis (4 center dot 5%) comprise 27 center dot 9% of the upregulated genes. Conclusions The synergy of resveratrol and alcohol against H. pylori was found in this study. The underlying mechanism of action of resveratrol against H. pylori may be mainly attributed to its inhibitory effect on translation, OMPs, transports, ATP synthase and possible oxidative damage. Significance and Impact of this Study Our study provides a global insight into the anti-H. pylori mechanism of resveratrol. Both resveratrol and alcohol can contribute to inhibition of ribosomes, changes in OMPs and oxidative damage, which may be the explanations of synergistic effect against H. pylori elicited by resveratrol and alcohol.