Applied Biochemistry and Biotechnology, Vol.190, No.1, 138-147, 2020
Peroxidase Immobilized Cryogels for Phenolic Compounds Removal
In this presented work, preparation of poly(AAm) cryogel, peroxidase immobilization onto the poly(AAm) cryogel, and usability of these enzyme modified cryogels for phenolic compounds removal were described. For this purpose, poly(AAm) cryogels were synthesized by using cryocopolymerization technique at sub-zero temperatures, and covalently functionalized with peroxidase enzyme by EDC/NHS chemistry. Characterization of the cryogels was carried out by FTIR, SEM, and EDX analysis. Maximum peroxidase loading onto poly(AAm) cryogel was found to be as 127.30 mg/g cryogel. Kinetic parameters of free and immobilized peroxidases were also investigated along with the stability tests. Finally, phenolic compounds removal efficiency of the peroxidase immobilized poly(AAm) cryogel was studied towards model phenolics such as phenol, bisphenol A, guaiacol, pyrogallol, and catechol; and very high phenolic removal efficiency was recorded.