Renewable Energy, Vol.145, 215-221, 2020
Improving cellulase recycling efficiency by decreasing the inhibitory effect of unhydrolyzed solid on recycled corn stover saccharification
Recycling enzymes associated with unhydrolyzed solid residue offers a potential strategy to reduce the amount of enzyme used during production of biofuels from lignocellulosic biomass. In this work, the hydrolytic efficiency of the enzymes associated with unhydrolyzed corn stover residue is evaluated and strategies to enhance their recycling efficiency are developed. We found whether direct recycling of corn stover residue could boost the recycling hydrolysis was dependent on the dosage of fresh cellulases. Under the investigated conditions, the addition of 5 FPU/g DM cellulases and BSA (2.5 mg/mL) could take the most advantage of the bound enzymes and suppress the negative effects caused by recalcitrant solid residues. The enzyme loading could be reduced by approximately 50% to achieve the same glucose yields (approximately 85%). This provided a highly promising technology for the optimization of solid residue recycling process and achieved the ultimate goal of cheaper and more efficient hydrolysis of lignocellulosic biomass. (C) 2019 Elsevier Ltd. All rights reserved.