화학공학소재연구정보센터
Journal of Bioscience and Bioengineering, Vol.128, No.4, 438-444, 2019
Nonnatural biosynthetic pathway for 2-hydroxylated xanthophylls with C-50-carotenoid backbone
Carotenoids are structurally diverse pigments with various important biological functions. There has been a large interest in the search for novel carotenoid structures, since only a slight structural changes can result in a drastic difference in their biological functions. Carotenoid-modifying enzymes show remarkable substrate promiscuity, allowing rapid access to a vast set of novel carotenoids by combinatorial biosynthesis. We previously constructed a nonnatural carotenoid biosynthetic pathway in Escherichia coli that can produce C-50 carotenoids having a longer chain than their natural C-40 counterparts. In this study, a carotenoid 2,2'-hydroxylase (crtG) from Brevundimonas sp. SD212 was coexpressed together with our laboratory-engineered C-50-zeaxanthin and C-50-astaxanthin biosynthetic pathways. We identified six novel nonnatural C-50-xanthophylls, namely, C-50-nostoxanthin, C-50-caloxanthin, C-50-adonixanthin, C-50-4-ketonostoxanthin, C-50-2-hydroxyastaxanthin, and C-50-2,2'-dihydroxyastaxanthin. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.