화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.517, No.3, 484-490, 2019
PRMT7 deficiency enhances adipogenesis through modulation of C/EBP-beta
Obesity that is critically correlated with the initiation and development of metabolic syndrome and cardiovascular diseases has increased worldwide. Adipogenesis is coordinated through multi-steps involving adipogenic commitment, mitotic clonal expansion (MCE) and differentiation. Recently, protein arginine methyltransferase 4 (PRMT4) and PRMT5 have been implicated in modulation of adipogenesis via regulation of C/EBP-beta activity or PPAR-gamma 2 expression. In the current study, we demonstrate a suppressive role of PRMT7 in adipogenesis. PRMT7-depleted preadipocytes or PRMT7(-/-) mouse embryonic fibroblasts (MEFs) displayed increased adipogenesis while PRMT7 overexpression attenuated it. PRMT7 depletion in preadipocytes promoted MCE, an initial step of adipogenesis. Furthermore, we found that PRMT7 interacted with and methylated a key adipogenic factor C/EBP-beta upon adipogenic induction and modulated the accumulation of C/EBP-beta at its target sites in the PPAR-gamma 2 promoter. Taken together, our data suggest that PRMT7 suppresses adipogenesis through modulation of C/EBP-beta activity. (C) 2019 Elsevier Inc. All rights reserved.