화학공학소재연구정보센터
Process Biochemistry, Vol.86, 58-64, 2019
Enhanced Asian sea bass skin defatting using porcine lipase with the aid of pulsed electric field pretreatment and vacuum impregnation
Lipid removal of Asian sea bass skin pretreated with pulsed electric field (PEF) at 24 kV/cm for 72 ms, followed by hydrolysis with porcine pancreas lipase (PPL) at 42.36 U/g skin dry matter with the aid of vacuum impregnation (VI) at various vacuum times (10, 15, 20 and 30 min) was investigated. The highest lipid reduction in PEF-treated skin was found at vacuum time of 20 min (p < 0.05). The efficacy of defatting was increased with increasing VI cycles (p < 0.05), in which lipids were eliminated by 97% when VI cycles of 4 were used. Also, the lipid content was lower than that found in sample treated with 30% isopropanol under the same VI condition (p < 0.05). Moreover, lipids extracted from PEF-VI-PPL treated skin had lower monounsaturated and polyunsaturated fatty acid contents as compared to other samples. Lower lipid distribution was noticeable in PEF-VIPPL treated skin. When PEF-VI-PPL-treated skin was hydrolyzed using papain at 0.3 U/g dry skin matter, weak fishy odor/flavor of the resulting hydrolyzed collagen was observed, compared with other samples (p < 0.05). Additionally, there was the reduced abundance of volatile compounds. Thus, the use of PPL in conjunction with VI could remove lipid in PEF-treated skin effectively.