Applied Chemistry for Engineering, Vol.30, No.5, 569-579, October, 2019
송화분의 초음파 파쇄 추출 방법에 따른 항염증 효능 및 간 보호 활성
Anti-inflammatory Efficacy and Liver Protective Activity of Pine Pollen according to Probe Sonicator Ultrasonic Disintegration Extraction Method
본 연구는 송화분을 probe sonicator (PS)로 초음파 파쇄하여 water, 70% ethanol, ethanol로 추출하여 항산화, 항염증, 간 보호 효과를 측정한 연구이다. 항염증 효과는 lipopolysaccharide (LPS)로 유도된 RAW264.7 세포에서 nitric oxide (NO) 및 cytokine 생성을 측정하였다. 70% ethanol-PS군에서 NO 저해율이 85.99 ± 0.12%로 가장 높게 나타났고 염증 관련 cytokine인 interlukin-1β (IL-1β), tumor necrosis factor-α (TNF-α)의 결과에서도 control군에 비해 약 63, 22%로 저해율로 우수한 효능을 보였다. 간 보호 효능은 HepG2 세포에 타크린을 처리하여 glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) 생성을 측정하였다. 70% ethanol-PS군에서 GOT, LDH의 결과에서 negative control군에 비해 약 28, 13%의 높은 저해율을 나타냈다. 따라서 송화분을 70% ethanol로 초음파 파쇄하여 추출하였을 때 항산화, 항염증, 간 보호 효과가 있는 기능성 식품 소재로서의 개발 가능성이 있을 것으로 사료된다.
In this study, the effect anti-oxidant, anti-inflammatory, and liver protective activity was investigated via quick ultrasonic disintegration of pine pollen using a probe sonicator (PS) followed by the extraction with water, 70% ethanol, and 100% ethanol. The anti-inflammatory effect was studied by measuring the production of nitric oxide (NO) and cytokine in RAW264.7 cells induced with lipopolysaccharides (LPS). The cell toxicity was also checked with an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the experiment was conducted using non-toxic 100 μg/mL. The NO inhibition rate was highest in the 70% ethanol PS group at 85.99 ± 0.12%. Also an excellent efficiency was obtained from the results of interlukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α), which is related to inflammation-related cytokine, with the respective inhibition rates of 63 and 22%. To examine liver protective activity, HepG2 cells were treated with Taclin, and the generation of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) was measured in the culture solution. From GOT and LDH generation results, the inhibition rates in the 70% ethanol PS group were 28% and 13%, respectively, which was higher compared to that of using negative control group. Our results suggest that pine pollen extracted in 70% ethanol using PS may be used to develop food products that have anti-aging, anti-inflammatory, and liver protective effects.
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