화학공학소재연구정보센터
Protein Expression and Purification, Vol.162, 32-37, 2019
Expression and characterization of albumin fusion protein canine IFN gamma-CSA in baculovirus-insect cell expression system
In this study, canine IFN gamma was fused by a flexible linker with canine serum albumin to construct the fusion protein IFN gamma-CSA for the purpose to design a long-acting canine IFN gamma. The fusion protein was successfully expressed in baculovirus-infected Sf9 insect cells and was purified by salting-out and ion exchange chromatography. The IFN gamma-CSA fusion possessed potent anti-viral assay against vesicular stomatitis virus in cultured cells. IFN gamma-CSA was also stable at 37 degrees C up to 72 h compared with 8 h for IFN gamma alone. In vivo pharmacokinetics demonstrated a significantly longer half-life for IFN gamma-CSA (15.42 h) than for canine reIFN gamma (1.51 h) in KM mice. These results indicate that IFN gamma-CSA expression in the baculovirus system was successful and provide a promising long-acting cytokine for veterinary clinical applications.